Self-Contained Biological Indicators consist of:
✅A plastic pipe and cap. The cap with a hole is placed on top of the pipe to allow the sterilizing agent to penetrate into the indicator. Between pipe and cap there is a filter paper which is used to avoid pollution.
✅A crushable media ampule which contains modified culture medium with a pH indicator. The modified culture medium will transition from the initial Blue color to Yellow in the presence of bacterial growth.
✅ An inoculated carrier of Bacillus atrophaeus ATCC 9372 is located at the bottom closed end of the pipe.
✅The Biological Indicator, when used with an incubator (incubation temperature 35-39°C), provides a biological culture result after 48 hours.
✅ The label on the outside of the plastic pipe contains a Type 1 chemicalindicator strip.
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